1.5 year-old, female, Angus cow (Bos taurus).This heifer was one of 200 head of Angus beef cattle that were rotated through a new pasture for 2 days
then moved back to previous pasture. Initially, 3 were found dead, and 3 sick cows, presenting weak and anorexic,
were hospitalized. The number of sick cows increased to 30, and 15 of these were reported dead within 3 days. Two
cows survived at the hospital and became icteric, with elevated liver enzymes.
At necropsy, the heifer was in good muscular condition.Â The liver was uniformly swollen and
pale, and on cut sections an enhanced reticular pattern was evident, somewhat resembling a nutmeg liver.Â Numerous
petechiae were noted within the epicardium and endocardium, but the hemorrhages did not extend into the subjacent
Liver: There is severe periacinar to massive hepatic necrosis.Â Periportal hepatocytes
are often spared, and form a bridging border around the necrotic foci.Â The hepatocytes are disassociated from their
neighboring hepatocytes, and have rounded up.Â There is moderate sinusoidal hemorrhage within the fields of the
disassociated hepatocytes.Â Sloughed cells admixed with necrotic debris and inflammatory cells accumulate within
the central vein lumens.
Liver: Hepatitis, periacinar to massive, acute, necrotizing, with
hepatocytic dissociation and sinusoidal hemorrhage.
Microcystin LR was detected in the rumen and cecal contents by liquid chromatography-mass
Microcystin LR (MCLR) is a microcyclic heptapeptide hepatotoxin, a secondary
metabolite that is produced during excessive growth and bloom of cyanobacterium (blue-green algae) in water
sources.Â Cyanobacterial bloom occurs most often in water that contains high concentrations of mineral nutrients,
especially phosphorus, and during times of sustained sun exposure with calm wind conditions.(3) After production,
cyanotoxins are often blown to the shallow regions and margins of the water source, effectively concentrating their
After ingestion of water contaminated with MCLR, the toxin is rapidly taken up in hepatocytes by carrier-mediated transport.Â Within the hepatocytes, MCLR inhibits protein phosphorylases 1 and 2A, which regulate cellular structural proteins.Â Inhibition of phosphorylases 1 and 2A promotes hyperphosphorylation of cytoskeletal proteins. Hepatocyte and endothelial cytoskeletal actin filaments distort and collapse, causing cellular structure alteration, detachment from adjoining hepatocytes and membrane instability.(1,2)
In early stages, periacinar to massive hepatic necrosis is represented by the detachment and rounding up of the altered hepatocytes and endothelial cells, extrusion of blood into the disrupted sinuses, with blood and necrotic, fully detached hepatocytes flowing into the central venous network.Â Hypovolemic shock due to hepatic hemorrhage usually causes death, although hypoglycemia and hyperkalemia from fulminant cessation of hepatic function can also be terminal events.(3) In cumulative low doses, liver failure, chronic inflammation and bilirubinemia can result in mortality.(3) If the animal survives the initial episode of hepatic damage, leukocytic inflammation within the periacinar necrosis proceeds to postnecrotic scarring, periacinar fibrosis, and irregular areas of parenchymal regeneration.(4)
Microcystin LR was detected in the rumen contents from this heifer, and additional heifers that died following exposure.Â Acute hepatic necrosis with hemorrhage was featured in the early cases that were submitted, and heifers that survived and later died exhibited subacute microscopic lesions.Â This incident occurred during late summer in Northern California, and site visits to the pasture where the affected heifers were housed found one water source that was a shallow, warm pond with brackish water and blue-green algae growth.
Liver: Necrosis, centrilobular to midzonal, diffuse, with hepatocellular dissociation, hemorrhage
and biliary hyperplasia.
Participants were approximately evenly divided between morphologic diagnoses of
necrotizing hepatitis and hepatocellular necrosis, and debated the merits of each during the conference session,
ultimately favoring the latter because of the absence of inflammation in the sections available for examination.
Massive hepatic necrosis indicates necrosis of entire acini and is present in some areas of the examined sections.
Submassive necrosis, with sparing of periportal hepatocytes, is prevalent.Â Using the acinar approach to hepatic
histology, the findings could be summarized as submassive necrosis involving acinar zones 2 and 3.
This case was also studied in consultation with the AFIP Department of Hepatic Pathology; their differential diagnosis for the histologic lesions was broad, and included viral infection, severe ischemia, metabolic disease, and acute autoimmune hepatitis, but a toxic etiology was favored.Â As detailed in the conference proceedings for WSC 2009-2010, Conference 16, case III, numerous plant toxins some of which are summarized in a table therein preferentially target centrilobular hepatocytes because of the extraordinary vulnerability of this cell population to hypoxia and their relatively high concentration of cytochromes P450.Â Therefore, the recognition of a centrilobular to midzonal distribution of hepatocellular necrosis, while essential to the development of an inclusive differential diagnosis, is not sufficient to definitively implicate a particular plant toxin.Â Additional evidence is required, and in this case, prominent hepatocellular dissociation is a key clue to prompt further investigation of MCLR as a possible etiology.Â Reiterating the importance of ancillary diagnostic tests, particularly in cases of suspected toxicosis, the detection of MCLR in ruminal and cecal contents by liquid chromatography-mass spectrometry solidifies the diagnosis in this case.Â The contributor provides an edifying review of the entity.
1.Â Batista T, deSousa G, Suput JS, Rahmani R, Suput D: Microcystin-LR causes the collapse of actin filaments in
primary human hepatocytes.Â Aquat Toxicol 65:85-91, 2003
2.Â Billiam M, Mukhi S, Tang L, Gao W, Wang JS: Toxic response indicators of microcystin-LR in F344 rats following a single-dose treatment.Â Toxicon 51:1068-1080, 2008
3.Â Briand JF, Jacquet S, Bernard C, Humbert JF: Health hazards for terrestrial vertebrates from toxic cyanobacteria in surface water ecosystems.Â Vet Res 34:361-377, 2003
4.Â Stalker MJ, Hayes MA: Liver and biliary system.Â In: Jubb, Kennedy, and Palmers Pathology of Domestic Animals, ed.Â Maxie MG, 5th ed., vol.Â 2, p.Â 327.Â Saunders Elsevier, Philadelphia, PA, 2007