Unknown age, male neutered domestic shorthair, feline (Felis cats).The cat had a clinical history of drooling for about two months. An oral examination
was made to determine the cause and a 1 x 2 x 2 cm growth was present on the upper gingiva
near the left incisor. Neoplasia or gingival hyperplasia was suspected. The mass was surgically
removed and submitted to the lab in 10% buffered formalin solution for histopathological
Grossly, the tissue contained a multilobulated mass.
The gingival submucosa is expanded by a poorly demarcated,
unencapsulated, multilobulated neoplasm. The neoplasm is composed of trabeculae, cords,
islands and nests of basaloid to cuboidal/columnar and polygonal cells supported on ample
fibrovascular stroma. The neoplastic cells occasionally exhibit a palisading pattern on the
periphery, often surround light eosinophilic amorphous to fibrillar material and centrally located
fragments of laminated keratin. The neoplastic cells have distinct cell borders, scant to ample
eosinophilic cytoplasm, and round to oval nuclei with finely stippled nuclear chromatin. The
nucleoli are variably distinct. Scattered mitotic cells (0-2/HPF) are observed. Multifocally, the
polygonal cells exhibit squamous differentiation. Multifocal areas of osteoid formation and
mineralization are evident in the mass. The light eosinophilic material stains positive for Congo
red dye, remains congophilic after treatment with potassium permanganate and exhibits applegreen
birefringence under polarized light.
Amyloid-producing odontogenic tumor (APOT).
Amyloid producing odontogenic tumor
Two broad groups of epithelial odontogenic tumors are recognized:
tumors lacking inductive properties on connective tissue and those having inductive properties
on connective tissue. Ameloblastoma and calcifying epithelial odontogenic tumors (amyloidproducing
odontogenic tumors) are considered non-inductive. In contrast, ameloblastic fibroma,
dentinoma, ameloblastic odontoma, complex odontoma, and compound odontoma have inductive
influence on the oral mesenchyme.(7) Amyloid-producing odontogenic tumor (APOT) is a rare
oral neoplasm reported in dogs and cats and contains variable amounts of amyloid deposition in
the neoplastic mass.(2) Amyloid is comprised of a heterogeneous group of proteins derived from
any of at least 25 different precursor molecules, and is considered a pathologic substance that
appears histologically as an extracellular, amorphous, congophilic protein with green
birefringence under polarized light.(5)
In humans, a neoplasm similar to amyloid producing odontogenic tumor in animals is named a calcifying epithelial odontogenic tumor (CEOT). Amyloid-producing odontogenic tumor was originally referred to as the counterpart of human CEOT. However, it was later described that APOT in dogs and cats is not a counterpart of CEOT in humans. Human CEOTs consist of sheets of eosinophilic epithelial cells showing considerable nuclear pleomorphism and invasive growth, whereas APOTs in animals mostly show basal cells with hyperchromatic nuclei, are arranged in palisades, and are benign masses that grow by expansion.(5) Due to such differences, amyloid-producing odontogenic tumor (APOT) was proposed as an appropriate alternative term for CEOTs in animals.(4)
Amyloid-producing odontogenic tumor is characterized by dental epithelium, with deposits of amyloid and sometimes prominent trabeculae of osteoid (dentinoid). The epithelium may be arranged in strands, nests or masses. Occasionally, there may be areas of mineralization of the epithelium or stroma in the form of small nodules or amorphous masses.(2) The distinctive features of calcifying epithelial odontogenic tumors in cats and dogs are the spherical amyloidlike deposits, which may undergo concentrically laminated calcification within the epithelial islands and stroma.(7)
It is believed that APOT passes through various developmental stages in which the neoplastic epithelium degenerates, forming amyloid globules which coalesce and calcify. However, the amyloid component seen in animal odontogenic tumors has not been thoroughly examined. Whether it is a secreted substance or a degenerative product remains controversial.(8) The neoplasm is suggested to originate from oral gingival epithelium or odontogenic epithelium within the connective tissue of the gingiva or within bone.(4) The amyloid in these tumors is suggested to be a secretory product of the neoplastic cells and possibly reflects an attempt to produce enamel by neoplastic ameloblasts.(1) Hirayama and co-workers examined the immunohistochemical profile of the amyloid protein from canine APOT using antibodies to ameloblastin, sheathlin, and amelogenin.(4) The neoplastic epithelial cells of APOT were focally reactive with antibodies to ameloblastin, sheathlin, amelogenin, and canine APOT amyloid. The similarity in amino acid sequence of the amyloid protein of canine APOT to that of enamel proteins, such as ameloblastin, sheathlin, and amelogenin, and the expression of these antigens in both APOT amyloid and in the neoplastic cells suggest that the amyloid of canine APOT is derived from enamel proteins secreted by ameloblasts. Based on these findings, the authors concluded that the precursor protein of amyloid fibrils in canine APOT may be derived from enamel proteins produced by ameloblasts and proposed that canine amyloid-producing odontogenic tumor (APOT) would more properly be named as canine amyloid-producing ameloblastoma (APA).(5) If this would apply to feline amyloid-producing odontogenic tumor remains to be determined.
In animals, almost all epithelial odontogenic tumors warrant a good prognosis. None has ever been reported to metastasize. They remain localized in the mandible or the maxilla, where they cause swelling and distortion. Complete surgical excision is usually curative. There is one exception to this rule: the calcifying epithelial odontogenic tumor (amyloid-producing odontogenic tumor) in cats and dogs, which although histologically benign is usually a locally invasive neoplasm and can cause destruction of bone and displacement of teeth.(7,8) Some cases were described to recur after excision.(4) In most cases, the neoplasms are considered as low grade malignancy and rarely metastasize.(8)
Amyloid-producing odontogenic tumor.
As the contributor discussed in an excellent overview of amyloidproducing
odontogenic tumors, recent studies have suggested the protein in canine APOTs is
derived from an ameoblastin-like peptide (AAmel), in contrast to the odontogenic amyloid
ameloblastic-associated protein (ODAM) found in human CEOTs.(3) In an even more recent
study, Delaney and co-workers analyzed the amyloid of three feline APOTs, and found the
amyloid from all three feline APOTs to contain an ameloblastin peptide identical to the AAmel
that had previously been identified in APOTs from a cat, a dog, and a tiger. Furthermore, the
presence of ameloblastin is consistent with the findings of a similar enamel protein in the
amyloid of APOTs from a Shih Tzu and eight other dogs, all of which were immunoreactive to
rat ameloblastin, porcine amelogenin and sheathlin; however, unlike their canine counterparts,
neither the feline amyloid nor the feline neoplastic epithelium exhibited positive
immunoreactivity for amelogenin. Ameloblastin (formerly called sheathlin) is an enamel matrix
protein that maintains the differentiation state of the ameloblast and is essential for enamel
formation. Ameloblastin and amelogenin (another enamel protein) are both produced by
ameloblasts during amelogenesis. In addition to ameloblastin, the feline APOTs in this study
also showed positive immunoreactivity with laminin antibodies. Laminins are found in dental
basement membrane during early tooth development; hence its presence in the amyloid of
APOTs further suggests APOTs are ameloblastic in origin.(3)
Additionally, in Hirayama and co-workers study of canine APOTs, the neoplastic cells exhibited positive immunoreactivity for cytokeratins (CK) AE1/AE3, CK9 and CK14. In the feline study, neoplastic cells were positive for CK AE1/AE3, CK14 and CK19. CK14 and CK19 are type I intermediate filaments of odontogenic epithelium; however, they are not specific, as they are also expressed in inner and outer enamel epithelium, cells from the stellate reticulum, stratum intermedium, dental lamina and Serres rests of the developing tooth.(3)
Overall, these results suggest that ameloblasts are the cell of origin of canine and feline APOTs, and ameloblasts produce the amyloid found in APOTs. Furthermore, feline and canine APOTs both apparently produce a similar type of amyloid which is distinct from the ODAM found in human CEOTs.(3)
1. Abbott DP, Walsh K, Diters RW. Calcifying epithelial odontogenic tumor in three cats and a dog. J Comp Pathol. 1986;96:131-136.
2. Brown CC, Baker DC, Barker IK. Alimentary tract, oral cavity, amyloid producing odontogenic tumor. In: Grant M Ed. Jubb, Kennedy and Palmers Pathology of Domestic Animals. Vol. II, 5th ed. Philadelphia, PA: Saunders Elsevier; 2007:25.
3. Delaney MA, Singh K, Murphy CL, Solomon A, Nel S, Boy SC. Immunohistochemical and biochemical evidence of ameloblastic origin of feline amyloid-producing odontogenic tumors in cats. Vet Pathol. Published online 25 June 2012. http://vet.sagepub.com/content/early/2012/06/25/0300985812452583 Accessed 23 November 2012.
4. Gardner DG, Dubielzig RR, McGee EV. The so-called calcifying epithelial odontogenic tumour in dogs and cats (amyloid-producing odontogenic tumour). J Comp Pathol. 1994;111:221230.
5. Hirayama K, Miyasho T, Ohmachi T, Watanabe T, Yokota H, Taniyama H. Biochemical and immunohistochemical characterization of the amyloid in canine amyloid-producing odontogenic tumor. Vet Pathol.2010;47(5)915-922.
6. Kuwamura M, Kanehara T, Yamate J, Shimada T, Kotani T. Amyloid-producing odontogenic tumor in a Shih-Tzu dog. J Vet Med Sci. 2000;62(6):655657.
7. Poulet FM, Valentine BA, Summers BA. A survey of epithelial odontogenic tumors and cysts in dogs and cats. Vet Pathol. 1992;29:369380.
8. Tsai YC, CR. Jeng, YX Zhuo, YC. Tsai, CH. Liu, VF Pang. Amyloid-producing odontogenic tumor and its immunohistochemical characterization in a Shih Tzu dog. Vet Pathol. 2007;44:233236.