JPC SYSTEMIC PATHOLOGY
HEMOLYMPHATIC SYSTEM
April 2018
H-N03

 

 

SIGNALMENT (#4007069):  Tissue from a 13 month old female CD-1 mouse

HISTORY:  Sentinel mouse exposed to dirty bedding and aerosol exposure of potential pathogens. Gross necropsy findings included nodular splenomegaly, hepatomegaly, multiple intraabdominal masses, diffuse discoloration of the spleen and liver with beige foci, and beige discoloration of the kidney.

HISTOPATHOLOGIC DESCRIPTION:  Lymph node, renal:  Diffusely affecting 100% of this section, effacing the lymphoid follicles, causing loss of the corticomedullary architecture, and infiltrating the perinodal connective tissue is an unencapsulated, poorly demarcated, densely cellular neoplasm composed of pleomorphic round cells arranged in sheets on a preexisting fibrovascular stroma. Neoplastic cells have distinct cell borders, moderate to abundant amounts of pale, eosinophilic cytoplasm with round, oval, or reniform nuclei that contain clumped to marginated chromatin and one to two distinct nucleoli. Anisocytosis and anisokaryosis are moderate.  Mitoses average four per individual high power field and are occasionally bizarre. 

Kidney: Diffusely the cytoplasm of proximal convoluted tubular epithelial cells are filled with numerous 2-3 micron round, brightly eosinophilic droplets (hyaline droplets). Low to minimal numbers of scattered neutrophils, macrophages, lymphocyte, and plasma cells are present in the interstitium.

Adrenal gland: Beneath the capsules there are multifocal dense aggregates of proliferative spindle cells arranged in streams that partially extend into the cortex (subcapsular spindle cell proliferation).  At the corticomedullary junction there is a band of cells that have large amounts of vacuolated or amphophilic cytoplasm and have multiple nuclei (X-zone involution).

Urinary bladder: No significant lesions.

MORPHOLOGIC DIAGNOSIS:  1. Lymph node, renal: Histiocytic sarcoma, CD-1 mouse, rodent.

  1. Kidney, proximal convoluted tubules: Intracytoplasmic hyaline droplets, diffuse.
  2. Adrenal gland: Spindle cell proliferation, subcapsular, multifocal, mild.
  3. Adrenal gland, corticomedullary junction: Epithelial cell vacuolation and degeneration, diffuse, (X-zone involution).
  4. Urinary bladder: Normal.

CONDITION NAME: 1. Histiocytic sarcoma (HS)

  1. Subcapsular spindle cell proliferation
  2. X-zone involution

GENERAL DISCUSSION: 

PATHOGENESIS: 

TYPICAL GROSS FINDINGS: 

TYPICAL LIGHT MICROSCOPIC FINDINGS: 

ADDITIONAL DIAGNOSTIC TESTS:

DIFFERENTIAL DIAGNOSIS: 

Microscopic:

COMPARATIVE PATHOLOGY:

Rat:

 

Dog:  Immunohistochemical profile of each type is listed in the table below

Histiocytic proliferative disorders:

Felines:

            cats)

Piglet: single case report of a congenital cutaneous histiocytosis of non-Langerhans cell origin

 

Disease

Species

Cell of Origin

Immunophenotype

Positive

Negative

Histiocytoma

Dog

Langerhans cell

CD1a, Cd11c/CD18, E-cadherin, Iba1

CD204

Cutaneous Langerhans cell histiocytosis

Dog

Langerhans cell

CD1a, Cd11c/CD18, E-cadherin, Iba1     

CD204

Cutaneous histiocytosis

Dog

Activated interstitial DC

CD1a, CD4, CD11c/CD18, CD90 (Thy-1), Iba1

CD204

Systemic histiocytosis

Dog

Activated interstitial DC

CD1a, CD4, CD11c/CD18, CD90 (Thy-1), Iba1

CD204 (?)

Histiocytic sarcoma

Dog, cat

Interstitial DC

CD1a, CD11c/CD18, Iba1, CD204 (+/-)

 

Hemophagocytic histiocytic sarcoma

Dog, cat

Macrophage

CD1a (+/-), CD11d/CD18 (dog), CD204, Iba1

 

Feline progressive histiocytosis

Cat

Interstitial DC

CD1a, CD11c/CD18, CD5 (50%), CD204 (+/-), Iba1

 

Pulmonary Langerhans cell histiocytosis

Cat

Langerhans cell

CD1a, CD18, E-cadherin, CD204 (+/-), Iba1 (?)

 

Dendritic cell leukemia

Dog

Interstitial DC

CD1a, CD11c/CD18

 

*Modified from:

  1. Moore PF. Canine and feline histiocytic disease. In: Meuten DJ, ed. Tumors in Domestic Animals. 5th ed. Ames, IA: Wiley Blackwell; 2017:322-336.
  2. Moore PF. A review of histiocytic diseases of dogs and cats. Vet Pathol. 2014;51(1):167-184.

REFERENCES: 

Barthold SW, Griffey SM, Percy DH. Pathology of Laboratory Rodents and Rabbits. 4th Ames, IA: Wiley-Blackwell; 2016:103,110-111,167-168.

  1. Boes KM, Durham AC. Bone marrow, blood cells and the lymphoid/lymphatic system. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th ed. St. Louis, MO: Elsevier; 2017:795-796.
  2. Busch MDM, Reilly CM, Luff JA, Moore PF. Feline pulmonary Langerhans cell histiocytosis with multiorgan involvement. Vet Pathol. 2008;45(6):816-824.
  3. Caswell JL, Williams KJ. Respiratory system. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 2. 6th ed. St. Louis, MO: Elsevier; 2016:498-499.
  4. Constantino-Casas F, Mayhew D, Hoather TM, Dobson JM. The clinical presentation and histopathologic-immnuohistochemical classification of histiocytic sarcoma in the Flat Coated Retriever. Vet Pathol. 2011;48:764-771.
  5. Craig LE, Ditter KE, Thompson KG. Bones and joints. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 1. 6th ed. St. Louis, MO: Elsevier; 2016:159-160.
  6. Hao X, Fredrickson TN, Chattopadhyay SK, et al. The histopathologic and molecular basis for the diagnosis of histiocytic sarcoma and histiocyte-associated lymphoma of mice. Vet Pathol. 2010;47(3):434-445.
  7. Helie P, Kiupel M, Drolet R. Congenital cutaneous histiocytosis in a piglet. Vet Pathol. 2014;51(4):812-815.
  8. Marcinowska A, Constantino-Casas F, Williams T, Hoather T, Blacklaws B, Dobson J. T Lymphocytes in Histiocytic Sarcomas of Flat-Coated Retriever Dogs. Vet Pathol. 2017;54(4):605-610.
  9. Mauldin EA, Peters-Kennedy J. Integumentary system. In: Maxie MG Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 1. 6th ed. St. Louis, MO: Elsevier, 2016:729-730.
  10. Moore PF. A review of histiocytic diseases of dogs and cats. Vet Pathol. 2014;51(1):167-184.
  11. Moore PF. Canine and feline histiocytic disease. In: Meuten DJ, ed. Tumors in Domestic Animals. 5th ed. Ames, IA: Wiley Blackwell; 2017:322-336.
  12. Moore PF, Affolter VK. Canine hemophagocytic histiocytic sarcoma: A proliferative disorder of CD11d+ macrophages. Vet Pathol. 2006;43:632-645.
  13. Valli VEO, Kiupel M, Bienzle D. Hematopoietic system. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 3. 6th ed. St. Louis, MO: Elsevier; 2016:243-255.
  14. Van kuijk L, van Ginkel K, de Vos JP, et al. Peri-articular histiocytic sarcoma and previous joint disease in Bernese mountain dogs. J Vet Intern Med. 2013;27(2):293-299.
  15. Van Nieuwenhuijze AE, Coghill E, Gray D, et al. Transgenic expression of GM-CSF in T cells causes disseminated histiocytosis. Am J Pathol. 2014;184(1):184-199.


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