JPC SYSTEMIC PATHOLOGY
REPRODUCTIVE SYSTEM
January 2019
R-N03

Signalment (JPC #1357631):  A dog 

HISTORY:  Not provided

HISTOPATHOLOGIC DESCRIPTION:  Testicle:  Two neoplasms are present.  First, markedly expanding the testicle and compressing the adjacent seminiferous tubules is an 8 mm diameter, well-circumscribed, encapsulated, multilobulated, densely-cellular neoplasm composed of polygonal cells (interstitial cells) arranged in cords and vague nests supported by a fine fibrovascular stroma.  Neoplastic cells have fairly distinct cell borders, moderate amounts of eosinophilic granular or occasionally vacuolated cytoplasm with eosinophilic globules, and one round, central nucleus with finely-stippled chromatin and a prominent magenta nucleolus.  There is moderate anisocytosis and anisokaryosis.  The mitotic rate is less than 1 per 10 HPF. Rare intranuclear cytoplasmic invaginations are observed. Filling the lumen and replacing spermatogenic and Sertoli cells in approximately 30% of surrounding seminiferous tubules is a second neoplasm composed of round cells (germ cells) with distinct cell borders, scant to moderate amounts of eosinophilic granular cytoplasm, and large, round, vesiculate nuclei with coarse chromatin and 1-2 large prominent magenta nucleoli.  There is moderate anisocytosis and anisokaryosis with rare giant cells and frequent multinucleated cells with up to 7 nuclei.  The mitotic rate averages 2 per HPF, and there is scattered single cell necrosis.  Remaining non-neoplastic seminiferous tubules exhibit degeneration, characterized by irregular undulant basement membranes, decreased to absent spermatogenesis, and occasional spermatid multinuclear giant cells within lumina.

MORPHOLOGIC DIAGNOSES

  1. Testicle:  Interstitial cell tumor, breed unspecified dog, canine.
  2. Testicle: Seminoma, intratubular, multifocal.
  3. Testicle, seminiferous tubules: Germ cell atrophy, diffuse, moderate, chronic with aspermatogenesis. 

GENERAL DISCUSSION:

PATHOGENESIS:

TYPICAL CLINICAL FINDINGS:

TYPICAL GROSS FINDINGS:

TYPICAL LIGHT MICROSCOPIC FINDINGS:

ULTRASTRUCTURAL FINDINGS:

ADDITIONAL DIAGNOSTIC TESTS: 

DIFFERENTIAL DIAGNOSIS: 

COMPARATIVE PATHOLOGY:

REFERENCES:

  1. Agnew DW, MacLachlan NJ. Tumors of the genital systems. In: Meuten DJ, ed. Tumors in Domestic Animals, 5th ed. Ames, IA: John Wiley & Sons, Inc.; 2017:706-711.
  2. Banco B, Giudice C, Ghisleni G. Romussi S, Behar DK, Grieco V. Immunohistochemical study of mixed germ cell sex cord stromal tumors in 13 canine testes. J Comp Pathol. 2015;152:182-187.
  3. Barthold SW, Griffey SM, Percy DH. Pathology of Laboratory Rodents and Rabbits, 4th ed. Ames, IA: John Wiley & Sons, Inc.; 2016:122-124, 169-170.
  4. Foster RA. Male genital system. In: Maxie MG ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 3. 6th ed. St. Louis, MO: Elsevier; 2016:492-497.
  5. Foster RA. Male reproductive system. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th St. Louis, MO: Elsevier; 2017:1210-1212.
  6. Hara S, Morita R, Shiraki A, et al. Expression of protein gene product 9.5 and Sal-like protein 4 in canine seminomas. J Comp Pathol. 2014;151(1):10-18.


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