JPC SYSTEMIC PATHOLOGY
URINARY SYSTEM
January 2018
U-V01

Signalment  (JPC# 320229):  Leopard frog (Rana pipiens)

HISTORY:  Large abdominal mass found at necropsy.

HISTOPATHOLOGIC DESCRIPTION:  Kidney:  Effacing 90% of renal architecture and extending to submitted borders is a multilobular, unencapsulated neoplasm composed of polygonal cells arranged in variably sized (up to 500 µm in diameter), irregularly shaped tubules and papillary projections, supported by a fine fibrovascular stroma.  Neoplastic cells are closely packed, cuboidal to columnar with variably distinct cell borders, and contain a moderate amount of eosinophilic, granular cytoplasm.  The neoplastic cells frequently pile up to 4 to 10 cell layers deep.  Nuclei are round to oval, centrally to basally positioned, with coarsely stippled chromatin and up to two variably indistinct nucleoli.  Mitotic figures are fewer than 1 per 10 high powered fields.  There are numerous round, up to 15 um in diameter, eosinophilic, intranuclear viral inclusion bodies that are peripheralize the chromatin.  Neoplastic structures are occasionally cystic and contain variable amounts of flocculent eosinophilic material.  Multifocally, remaining tubules are ectatic and lined by attenuated epithelium and contain sloughed epithelial cells and debris.  Within remaining parenchyma, there is a mild, multifocal lymphocytic inflammation and minimal hemorrhage.   

MORPHOLOGIC DIAGNOSIS:  Kidney:  Renal adenocarcinoma, with eosinophilic intranuclear viral inclusions, Northern leopard frog (Rana pipiens), amphibian.

ETIOLOGIC DIAGNOSIS:  Ranid herpesvirus renal adenocarcinoma

CAUSE:  Ranid herpesvirus (RaHV-type1)

CONDITION:  Lucké's tumor of leopard frogs, Lucké’s renal adenocarcinoma

GENERAL DISCUSSION: 

PATHOGENESIS: 

TYPICAL CLINICAL FINDINGS: 

TYPICAL GROSS FINDINGS: 

TYPICAL LIGHT MICROSCOPIC FINDINGS: 

DIFFERENTIAL DIAGNOSIS:

COMPARATIVE PATHOLOGY:

Adapted from MacLachlan, Dubovi (2017)

Viruses that can induce tumors in Domestic or Laboratory Animals or Humans

Family/Genus
DNA Viruses
Virus Type of Tumor
Poxviridae/Leporipoxvirus Rabbit and squirrel fibroma virus Fibromas and myxomas
Poxvirinae/Yatapox Yaba monkey tumor virus Histiocytoma in monkeys
Herpesviridae: Alphaherpesvirinae Marek’s disease virus T cell lymphoma in fowl
Gammaherpesvirinae Epstein-Barr virus
Baboon herpesvirus
Cottontail rabbit herpesvirus
B cell lymphoma, etc
Lymphoma in baboons
Lymphoma in rabbits
Alloherpesviridae Lucke frog herpesvirus Renal adenocarcinoma in frogs
Papillomaviridae Bovine papillomavirus 4
Bovine papillomavirus 7
Cottontail rabbit papillomavirus
Equine papillomavirus
Papillomas, carcinoma of intestine/bladder
Papillomas, carcinoma of eye
Papillomas, skin cancer
Squamous cell carcinoma
Polyomaviridae/Polyomavirus Murine polyomavirus Tumors in newborn rodents Central nervous system

Viruses that can induce tumors in Domestic or Laboratory Animals or Humans

Hepadnaviridae/
Orthohepadnavirus
Woodchuck hepatitis virus Hepatocellular carcinoma
Hepadnaviridae/
Avihepadnavirus
Duck hepatitis virus Hepatocellular carcinoma
Retroviridae/
Alpharetrovirus
Avian Leukosis virus
Rous sarcoma virus
Avian myeloblastosis virus
Lymphoma, leukemia, osteopetrosis, nephroblastoma in fowl
Sarcoma in fowl
Myeloblastosis in fowl
Betaretrovirus Mouse mammary tumor virus
Ovine pulmonary
adenocarcinoma virus (jaagsiekte virus)
Mammary carcinoma
Pulmonary adenocarcinoma sheep
Gammaretrovirus Feline Leukemia virus
Feline sarcoma virus
Murine leukemia/sarcoma virus
Leukemia
Sarcoma in cats
Leukemia, lymphoma, sarcoma
Deltaretrovirus Avian reticuloendotheliosis virus
Bovine Leukemia virus
Reticuloendotheliosis in fowl
B cell lymphoma, leukemia

References: 

  1. Carlson DL, McKinnell RG, Rollins-Smith LA, Sauerbier W. The presence of DNA sequences of the Lucké herpesvirus in normal and neoplastic kidney tissue of Rana pipiens. J Comp Pathol. 1994;110(4):349-55.
  2. O’Rourke DP, Schultz TW. In: Anderson LC, Fox JG, Loew FM, Quimby FW, eds. Laboratory Animal Medicine. 2nd ed. London, England; Academic Press; 2002:817-818.
  3. Granoff A. Herpesvirus and the Lucké tumor. Cancer Res. 1973; 33(6):1431-1433.
  4. Green DE, Harshbarger JC. Spontaneous neoplasia in amphibia. In: Wright KM, Whitaker BR, eds. Amphibian Medicine and Captive Husbandry. Malabar, FL: Krieger Publishing; 2001:365-368.
  5. Lucke´ B. Carcinoma in the leopard frog: its possible causation by a virus. J Exp Med. 1938; 68(4):457–468.
  6. MacLachlan JN, Dubovi EJ, eds. Fenner’s Veterinary Virology. 5th ed.  London, UK: Academic Press; 2017:77, 191, 213, 215.
  7. Origgi FC, et al. Ranid herpesvirus 3 and proliferative dermatitis in free-ranging wild common frogs (Rana temporaria). Vet Pathol. 2017;54(4):686-694.
  8. Skinner MS, Mizell M. The effects of different temperatures on herpesvirus induction and replication in Lucké’s tumor explants. Lab Invest. 1972;26(6):671.


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