JPC SYSTEMIC PATHOLOGY
REPRODUCTIVE SYSTEM
January 2019
R-B03

SIGNALMENT:  4-year old male bison

HISTORY:  Slide A: This bison had strong positive titers for Brucella abortus, and at slaughter, inspectors noted a testicle twice normal size

HISTOPATHOLOGIC DESCRIPTION:  Testis and epididymis:  Effacing 80% of the normal architecture and compressing, separating, surrounding and replacing seminiferous tubules are multifocal to coalescing areas of caseonecrotic debris admixed with degenerate neutrophils, intra and extracellular 1x2 um coccobacilli, and foci of mineral that are further surrounded by epithelioid macrophages, multinucleate giant cells, fewer lymphocytes, and reactive fibroblasts (coalescing granulomas).  These areas are further surrounded by granulation tissue and abundant collagen (fibrosis) forming thick bands which dissect between granulomas. The inflammatory infiltrate and fibrosis extends into the adjacent tunica albuginea and epididymis. There is diffuse germ cell atrophy with lack of spermatids in the remaining seminiferous tubules.

MORPHOLOGIC DIAGNOSIS:  Testis and epididymis: Granulomas, multiple, with fibrosis, germ cell atrophy and aspermia and numerous coccobacilli, Bison (Bison bison), bovine.

SIGNALMENT (JPC #1713947):  Bovine fetus

HISTORY:  Slide B:  This fetus was aborted

HISTOPATHOLOGIC DESCRIPTION:  Lung: Multifocally and randomly affecting 50% of the lung, filling alveolar spaces, and adjacent small bronchioles and expanding alveolar septa are nodular aggregates of numerous macrophages and degenerate neutrophils, few multinucleated giant cells, lymphocytes, plasma cells, abundant fibrin, edema, and hemorrhage. There is multifocal alveolar septal necrosis, with replacement by a similar population of inflammatory cells, necrotic debris and fibrin. The pleura, interlobular septa and associated lymphatics are expanded by moderate edema, fibrin and hemorrhage and small aggregates of macrophages, lymphocytes and plasma cells.

Liver: Multifocally infiltrating portal areas and randomly scattered throughout the parenchyma are moderate numbers of lymphocytes and plasma cells, with fewer neutrophils and macrophages. There is multifocal extramedullary hematopoiesis.

MORPHOLOGIC DIAGNOSIS:

  1. Lung: Bronchopneumonia, pyogranulomatous and fibrinonecrotic, multifocal to coalescing, moderate, with interlobular edema, and pleuritis, breed unspecified, bovine.
  2. Liver: Hepatitis, periportal and random, lymphoplasmacytic, multifocal, mild.

SIGNALMENT (JPC #2020923):  Goat

HISTORY:  Slide C: This goat had aborted

HISTOPATHOLOGIC DESCRIPTION:  Placenta (placentome): Multifocally and extensively filling caruncular crypts and effacing chorionic villi are large areas of lytic necrosis, characterized by loss of architecture and replacement by eosinophilic cellular and karyorrhectic debris admixed with numerous degenerate neutrophils and macrophages and prominent colonies 1x2 um basophilic coccobacilli. Bacteria are also present within macrophages, cytotrophoblasts, and syncytiotrophoblasts.  Multifocally less affected villi contain necrotic multinucleated syncytiotrophoblasts (with shrunken hypereosinophilic cytoplasm and pyknotic nuclei) and/or are multifocally denuded. Diffusely, the lamina propria of the caruncle and intercotyledonary area of the placenta is edematous and expanded by mucin and low numbers of neutrophils and macrophages.  Caruncular vessel walls within are infiltrated by similar inflammatory cells, necrotic debris and fibrin (necrotizing vasculitis) and vessel lumina often contain fibrin thrombi.

MORPHOLOGIC DIAGNOSIS:  Placenta (placentome): Placentitis, necrotizing, acute, focally extensive, severe, with necrotizing vasculitis, fibrin thrombi and numerous intratrophoblastic and extracellular colonies of coccobacilli, breed unspecified, caprine.

ETIOLOGIC DIAGNOSIS:  Testicular, hepatic, pulmonary, and placental brucellosis

CAUSE:  Brucella abortus

CONDITION:  Brucellosis

SYNONYMS:  Undulant fever, Bang's disease

GENERAL DISCUSSION:

PATHOGENESIS:

TYPICAL CLINICAL FINDINGS:

TYPICAL GROSS FINDINGS: 

TYPICAL LIGHT MICROSCOPIC FINDINGS:

ADDITIONAL DIAGNOSTIC TESTS: 

DIFFERENTIAL DIAGNOSIS:  

Abortion:

Bovine:

Ovine / Caprine:

Swine: causes of abortion, stillbirth, and/or mummification

Orchitis:

Bovine:

Caprine / Ovine:

Feline:

Swine:

Equine:

Canine:

Epididymitis:

Bovine:

Ovine/Caprine:

Swine:

Canine:

Equine:

Feline:

COMPARATIVE PATHOLOGY:

REFERENCES:

  1. Buckle K, Roe WD, Howe L, Michael S, Duignan PJ, Burrows E, Ha HJ, Humphrey S, McDonald WL. Brucellosis in endangered Hector's dolphins (Cephalorhynchus hectori). Vet Pathol. 2017 Sep;54(5):838-845.
  2. Burrough ER, Wu Z, Sahin O, Zhang Q, Yaeger MJ. Spatial distribution of putative growth factors in the guinea pig placenta and the effects of these factors, plasma, and bile on the growth and chemotaxis of Campylobacter jejuni. Vet Pathol. 2012 May; 49(3):470-81.
  3. Buergelt CD. Color Atlas of Reproductive Pathology of Domestic Animals. St Louis, MO: Mosby-Year Book, Inc.; 1997:38-9, 62-3, 171-3, 179-93.
  4. Duncan CG, Tiller R, Mathis D, Stoddard R, Kersh GJ, Dickerson B, Gelatt T. Brucella placentitis and seroprevalence in northern fur seals (Callorhinus ursinus) of the Pribilof Islands, Alaska. J Vet Diagn Invest. 2014 Jul;26(4):507-512.Figueiredo P, Ficht TA, Rice-Ficht A, Rossetti CA, Adams LG. Pathogenesis and immunobiology of Brucellosis review of Brucellae-host interactions. Am J. Pathol. 2015;185(6):1505-17.
  5. Foster RA. Female reproductive system and mammae. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th ed. St. Louis, MO: Elsevier; 2017:1180,1182-1183,1188,1191.
  6. Foster RA. Male genital system. In: Maxie MG, ed. Jubb, Kennedy, and Palmers Pathology of Domestic Animals. Vol 3. 6th ed. St Louis, MO: Elsevier; 2016:483-499.
  7. Foster RA. Male reproductive system. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th ed. St. Louis, MO: Elsevier; 2017:1210.
  8. Glynn KM, Lynn TV. Brucellosis. J Am Vet Med Assoc. 2008; 233(6):900-08.
  9. Gonzalez-Barrientos R, et al. Pathology of striped dolphins (Stenella coeruleoalba) infected with Brucella ceti. J Comp Path. 2010 May; 142(2):347-52.
  10. Kirkwood RN, Althouse GC, Yaeger MJ, Carr J, Almond GW. Diseases of the reproductive system. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW, eds., eds. Diseases of Swine. 10th ed. Ames, IA: Blackwell Publishing; 2012:155-160.
  11. McCollum M, Rhyan J, Coburn S, Ewalt D et al. Clinical, culture, serology, and histopathology outcomes of bighorn sheep experimentally infected with Brucella ovis. J Wildl Dis. 2013; 49(4):900-10.
  12. Olson SC, Palmer MV. Advancement of knowledge of Brucella over the past 50 years.  Vet Pathol. 2014; 51(6):1076-1089.
  13. Pedersen K, Quance CR, Robbe-Austerman S, Piaggio AJ, et al. Identification of Brucella suis from feral swine in the selected states in the USA. J Wildl Dis. 2014;50(2):171-179.
  14. Samartino CG, et al. Brucella abortus induces the secretion of proinflammatory mediators from glial cells leading to astrocyte apoptosis. Am J Pathol. 2010; 176(3):1-16.
  15. Schlafer DH, Foster RA. Female genital system. In: Maxie MG, ed. Jubb, Kennedy, and Palmers Pathology of Domestic Animals. Vol 3. 6th ed. St. Louis, MO: Saunders Elsevier; 2016:402-406.
  16. Seleem MN, Boyel SM, Sriranganathan N. Brucellosis: A re-emerging zoonosis. Vet Micro. 2010 Jan 27; 140(3-4):392-8.
  17. Zachary JF. Mechanisms of microbial infections. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th ed. St. Louis, MO: Elsevier; 2017:182-184,192-193. 


Click the slide to view.



Click on image for diagnostic series.



Back | Home | Contact Us | Links | Help |