JPC SYSTEMIC PATHOLOGY
HEMOLYMPHATIC SYSTEM
April 2018
H-F01

 

Signalment (JPC# 1535256):  Pacific white-sided dolphin (Lagenorhynchus obliquidens).

HISTORY:  Tissue from a dolphin, which had been in captivity for three years.

HISTOPATHOLOGIC DESCRIPTION:  Lymph node:  Diffusely replacing and effacing normal lymphoid architecture within the cortex, paracortical areas and extending into the medulla are numerous macrophages, viable and degenerate neutrophils, fewer lymphocytes and plasma cells, fibrosis and abundant eosinophilic fibrillar material (fibrin) and clear space (edema). Multifocally there are large areas of eosinophilic cellular and karyorrhectic debris (necrosis) and hemorrhage.  Admixed are numerous 2 x 6 µm, round to oval to elongate (cigar shaped), intrahistiocytic and extracellular yeast that are surrounded by a 1 - 3 µm clear halo.  Rarely, individual yeasts are encircled by numerous eosinophilic radiating spicules of Splendore-Hoeppli material (asteroid bodies).  Multifocally the walls of both arterioles and veins are expanded by neutrophils and macrophages, fibrin and necrotic cellular debris (vasculitis).  Diffusely the lymph node capsule is expanded by neutrophils, macrophages, fewer lymphocytes and plasma cells admixed with fibrin, edema, hemorrhage and cellular and karyorrhectic debris (capsulitis).

H-F01b:  Lymph node (GMS):  Diffusely there are numerous intrahistiocytic and extracellular round to oval to elongate, 2 x 6 µm (cigar shaped) yeasts, often with multiple buds. Admixed are many fungal hyphae that are 4-6 um thick with parallel cell walls, irregular septation and right angle, and dichotomous branching (postmortem overgrowth).

 

MORPHOLOGIC DIAGNOSIS: Lymph node: Lymphadenitis and capsulitis, pyogranulomatous and necrotizing, diffuse, severe, with vasculitis and intrahistiocytic and extracellular fungal organisms, Pacific white-sided dolphin (Lagenorhynchus obliquidens), cetacean.

ETIOLOGY: Sporothrix schenckii

ETIOLOGIC DIAGNOSIS: Sporothricotic lymphadenitis

GENERAL DISCUSION: 

PATHOGENESIS:

TYPICAL CLINICAL FINDINGS: 

TYPICAL GROSS FINDINGS: 

TYPICAL LIGHT MICROSCOPIC FINDINGS: 

ADDITIONAL DIAGNOSTIC TESTS: 

DIFFERENTIAL DIAGNOSIS:

COMPARATIVE PATHOLOGY: 

 REFERENCES:

  1. Boes KM, Durham AC. Bone marrow and blood cells and the lymphoid/lymphatic system. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th St. Louis, MO: Elsevier; 2017: 782, E-Table 13-2
  2. Crothers SL, White SD, Ihrke PJ, et al. Sporotrichosis: a retrospective evaluation of 23 cases seen in northern California (1987-2007). Vet Dermatol. 2009; 20(4):249-259
  3. Hargis AM, Myers S. The integument. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th St. Louis, MO: Elsevier; 2017: 1083
  4. Mauldin EA, Peters-Kennedy J. Integumentary System.  In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 1. 6th St. Louis, MO: Elsevier; 2016:655-657
  5. Miranda LH, Quintella LP, Santos IB, et al. Comparative histopathological study of sporotrichosis and American tegumentary leishmaniasis in dogs from Rio de Janeiro. J Comp Pathol. 2010;143(1):1-7
  6. Murphy H. Great apes. In: Miller RE, Folwer ME, eds. Fowler’s Zoo and Wild Animal medicine. Vol 8. St. Louis, MO: Elsevier; 2015: 351
  7. Pohlman LM, Bagladi-Swanson MS, Torres-Irizarry MS. Pathology in practice. Disseminated pyogranulomatous inflammation with intralesional sporothrix organisms in a cat. J Am Vet Med Assoc. 2014; 245(2):187-189.

 


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