April 2018
H-P07 (NP)

SIGNALMENT (JPC# 1752289):  Dog

HISTORY:  This dog had splenomegaly and hepatomegaly.

HISTOPATHOLOGIC DESCRIPTION:  (H-P07a) Spleen:  Diffusely splenic red pulp is effaced by numerous macrophages, moderate numbers of plasma cells, fewer lymphocytes, and occasional neutrophils that fill and expand the cords and red pulp vascular spaces, and replace and obscure the white pulp, trabeculae, and capsule.  Macrophages are up to 40 um in diameter and contain numerous 2-4 um, ovoid, intracytoplasmic amastigotes with an eccentric, round to ovoid, basophilic nucleus and a <1 um, linear, basophilic, kinetoplast.  Multifocally, there are scattered random areas of karyorrhectic and cellular debris (lytic necrosis) containing numerous viable and degenerate neutrophils admixed with variable amounts of an eosinophilic beaded to fibrillar fibrin.  There is multifocal histiocytic erythrophagocytosis and hemosiderosis, and rare megakaryocytes.

(H-P07a) Liver:  Diffusely sinusoids, portal and periportal areas, and vessels are expanded by numerous macrophages, lymphocytes, plasma cells, and fewer neutrophils.  Most macrophages are filled with protozoal organisms as previously described in the spleen.  Diffusely, there is centrilobular to midzonal disruption, attenuation and loss of hepatic cords.  In these areas hepatocytes are rarely degenerate or necrotic, there are increased numbers of hemosiderin laden Kupfer cells, and central veins occasionally contain fibrin thrombi.

(H-P07b) Price Giemsa:  Intracytoplasmic amastigotes have bright blue nuclei, purple kinetoplasts and light blue cytoplasm.


  1. Spleen:  Splenitis, histiocytic and plasmacytic, subacute, diffuse, severe, with multifocal necrosis and numerous intrahistiocytic protozoal amastigotes, breed unspecified, canine.
  2. Liver: Hepatitis, histiocytic, subacute, diffuse, moderate, with multifocal hepatocellular degeneration, necrosis and loss, and numerous intrahistiocytic protozoal amastigotes.

ETIOLOGIC DIAGNOSIS:  Splenic and hepatic leishmaniasis

CAUSE:  Leishmania spp. (differentials include L. infantum/chagasi, L. donovani)

CONDITION:  Visceral leishmaniosis

SYNONMYNS:  Kala-azar, Dum dum fever













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