JPC SYSTEMIC PATHOLOGY
HEMOLYMPHATIC SYSTEM
March 2018
H-B03 (NP)

Signalment (JPC #822016):  Unknown age cow

HISTORY:  A cow with chronic diarrhea

HISTOPATHOLOGIC DESCRIPTION:  Lymph node: Multifocally, effacing up to 40% of both cortex and medulla are coalescing areas of lytic necrosis, with loss of normal architecture and replacement by eosinophilic cellular and karyorrhectic debris, admixed with fibrin and hemorrhage. Multifocally there is lymphcytolysis and low numbers of viable and degenerate neutrophils, surrounded by aggregates of foamy macrophages and few multinucleate giant cells of both Langhans and foreign body type that extend into and fill cortical and medullary sinuses.  Macrophages and giant cells often contain indistinct negative staining 0.3 x 1.5 µm intracytoplasmic bacilli and golden-brown granular pigment (hemosiderin).  Sinuses also contain low numbers of plasma cells and neutrophils and individual necrotic cells. 

Acid fast stain: There are numerous acid fast 0.3 x 2 µm bacilli within macrophages and free within sinuses and necrotic debris.

MORPHOLOGIC DIAGNOSIS:  Lymph node: Lymphadenitis, necrotizing and pyogranulomatous, multifocal, marked with numerous acid fast intrahistiocytic bacilli, breed unspecified, bovine.

ETIOLOGIC DIAGNOSIS:  Mycobacterial lymphadenitis

CAUSE:  Mycobacterium avium subsp. Paratuberculosis (Map)

CONDITION:  Johne"s disease, paratuberculosis

GENERAL DISCUSSION: 

PATHOGENESIS:

TYPICAL CLINICAL FINDINGS:

TYPICAL GROSS FINDINGS:

TYPICAL LIGHT MICROSCOPIC FINDINGS:

DIFFERENTIAL DIAGNOSIS:

Lymphadenitis

COMPARATIVE PATHOLOGY:

REFERENCES:

  1. Ackermann MR. Inflammation and healing. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th St. Louis, MO: Elsevier; 2017: 111.
  2. Burgess TL, Witte CL, Rideout BA. Early-life exposures and Johne"s disease risk in zoo ruminants. J Vet Diagn Invest. 2018; 30(1):78-85.
  3. Delgado L, Garcia Marin JF, Munoz M, et al. Pathological findings in young and adult sheep following experimental infection with 2 different doses of Mycobacterium avium subspecies paratuberculosis. Vet Pathol. 2013; 50(5):857-866.
  4. Fernández M, Benavides J, Castaño P, et al. Macrophage subset.s within granulomatous intestinal lesions in bovine paratuberculosis. Vet Pathol. 2017; 54(1):82-93
  5. Forde T, Kutz, S, De Buck J, et al. Occurrence, diagnosis, and strain typing of Mycobacterium avium subspecies paratuberculosis infection in Rocky Mountain bighorn sheep (Ovis canadensis canadensis) in southwestern Alberta. J Wildl Dis. 2012; 48(1): 1–11.
  6. Gelberg HB. Alimentary system and the peritoneum, omentum, mesentery, and peritoneal cavity. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th St. Louis, MO: Elsevier; 2017: 333, 398-399.
  7. Gulliver EL, Plain KM, Begg DJ, et al. Histopathological characterization of cutaneous delayed-type hypersensitivity and correlations with intestinal pathology and systemic immune responses in sheep with paratuberculosis. J Comp Pathol. 2015; 153(2-3):67-80.
  8. Kruger C, Kohler H, Liebler-Tenorio EM. Sequential development of lesions 3, 6, 9, and 12 months after experimental infection of goat kids with Mycobacterium avium Paratuberculosis. Vet Pathol. 2015; 52(2):276-290.
  9. Masters NJ, Flach E. Tragulidae, Moschidae, and Cervidae. In: Miller RE, Folwer ME, eds. Fowler’s Zoo and Wild Animal medicine. Vol 8. St. Louis, MO: Elsevier; 2015: 619, 621.
  10. Miller MA, Buss PE. Rhinoceridae (Rhinoceroses). In: Miller RE, Folwer ME, eds. Fowler’s Zoo and Wild Animal medicine. Vol 8. St. Louis, MO: Elsevier; 2015: 542, 546.
  11. Miranda C, Matos M, Pires I, et al. Diagnosis of Mycobacterium avium complex in granulomatous lymphadenitis in slaughtered domestic pigs. J Comp Path. 2012; 147(4): 401-405.
  12. Plattner BL, Chiang YW, Roth JA, et al. Direct inoculation of Mycobacterium avium subspecies paratuberculosis into ileocecal Peyer’s patches results in colonization of the intestine in a calf model.  Vet Pathol. 2012; 48(3): 584-592.
  13. Robveille C, Albaric O, Gaide N, et al. Disseminated mycobacteriosis manifesting as paraplegia in two Parma wallabies (Macropus parma) naturally exposed to Mycobacterium avium. J Vet Diagn Invest. 2015; 27(6): 767-771.
  14. Smith SL, Wilson PR, Collett MG, et al. Liver biopsy histopathology for diagnosis of Johne’s disease in sheep. Vet Pathol. 2014; 51(5):915-918.
  15. Uzal FA, Plattner BL, Hostetter JM. Alimentary System. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 2. 6th St. Louis, MO: Elsevier; 2016:100, 115, 194-197.
  16. Valli VEO, Kiupel M, Bienzle D. Hematopoietic System In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 3. 6th St. Louis, MO: Elsevier; 2016:204-205.
  17. Vogelnest L. Marsupialia (Marsupials). In: Miller RE, Folwer ME, eds. Fowler’s Zoo and Wild Animal medicine. Vol 8. St. Louis, MO: Elsevier; 2015: 266-267.
  18. Wolfe BA. Bovidae (except sheep and goats) and Antilocapridae. In: Miller RE, Folwer ME, eds. Fowler’s Zoo and Wild Animal medicine. Vol 8. St. Louis, MO: Elsevier; 2015: 638-640.
  19. Zachary JF. Mechanisms of microbial infections. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th St. Louis, MO: Elsevier; 2017: 162-163.

 


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