JPC SYSTEMIC PATHOLOGY

INTEGUMENTARY SYSTEM

October 2016

I-N27

 

SLIDE A

Signalment (JPC 3044519):  Dog, breed and age unspecified

HISTORY: Tissue from a firm, 3 x 4 cm skin mass at left paralumbar fossa

HISTOPATHOLOGIC DESCRIPTION:  Haired skin:  Infiltrating the subcutis, separating, surrounding and replacing skeletal muscle, and extending to cut borders, is an unencapsulated, well demarcated, infiltrative, densely cellular neoplasm composed of round to spindle cells arranged in broad sheets on a moderate fibrovascular stroma. Neoplastic cells have variably distinct cell borders, moderate to abundant eosinophilic finely fibrillar to occasionally microvacuolated cytoplasm, a round, oval, or reniform nucleus with finely stippled chromatin, and one distinct nucleolus. The mitotic count is regionally variable with up to 3 per 400x field. There is moderate anisocytosis and anisokaryosis.  Multifocally, there are moderate numbers of neoplastic multinucleated giant cells measuring up to 100 µm in diameter with up to 30 nuclei.  There is scattered single cell necrosis as well as large areas of coagulative necrosis with retention of cellular architecture and loss of differential staining and numerous cholesterol clefts and eosinophilic and cellular debris. Multifocally there are hemosiderin-laden macrophages and cholesterol clefts.  Multifocally, moderate numbers of myofibers are deeply eosinophilic, rounded, shrunken, with loss of cross striations (atrophy) and rarely fragmented (necrosis). 

MORPHOLOGIC DIAGNOSIS:  Haired skin: Cutaneous histiocytic sarcoma, breed not specified, canine

SLIDE B

Signalment (JPC 1918993):  Cat, breed and age unspecified

HISTORY:  Tissue from a multilobulated mass in the lumbar region

HISTOPATHOLOGIC DESCRIPTION:  Haired skin and subcutis:  Infiltrating the subcutis and deep dermis, elevating the epidermis, and extending to cut borders, is an unencapsulated, densely cellular, multilobulated neoplasm composed of spindle cells arranged in long, interlacing streams and bundles on a moderate fibrovascular stroma; lobules are separated by broad bands of mature collagen. Neoplastic cells have indistinct borders and moderate amounts of eosinophilic, fibrillar cytoplasm.  Nuclei are elongate with finely stippled chromatin and have 1-2 variably distinct nucleoli. There is mild anisocytosis and anisokaryosis. Admixed with spindle cells are moderate numbers of more round, pleomorphic, multinucleated giant cells (up to 100um in diameter) with up to 10 nuclei and often prominent nucleoli. The mitotic count averages 3 per 10-400x fields.  Neoplastic cells surround a large, focally extensive area of drop-out and necrosis.  Multifocally within the neoplasm there are small areas of lytic necrosis with eosinophilic and cellular debris and hemorrhage. At the periphery of the neoplasm, often perivascularly, there are aggregates of lymphocytes with fewer plasma cells. The surrounding connective tissue and dermis are loosely arranged and expanded by clear space, with mildly ectatic lymphatics (edema). 

MORPHOLOGIC DIAGNOSIS:  Haired skin and subcutis:  Undifferentiated pleomorphic sarcoma, (previously known as malignant fibrous histiocytoma), breed not specified, feline

SYNONYMS:  Malignant fibrous histiocytoma, malignant giant cell tumor of soft parts, extraskeletal giant cell tumors, dermatofibrosarcomas, anaplastic sarcoma with giant cells

GENERAL:

 PATHOGENESIS:

Localized histiocytic sarcoma

Feline progressive histiocytosis

 TYPICAL CLINICAL FINDINGS: 

Localized histiocytic sarcoma

Feline progressive histiocytosis

 TYPICAL GROSS FINDINGS:

Localized histiocytic sarcoma

Feline progressive histiocytosis

 TYPICAL LIGHT MICROSCOPIC FINDINGS:

Localized histiocytic sarcoma

 Feline progressive histiocytosis

 ADDITIONAL DIAGNOSTIC TESTS: 

Localized histiocytic sarcoma

Feline progressive histiocytosis

 DIFFERENTIAL DIAGNOSIS:

Localized histiocytic sarcoma

Feline progressive histiocytosis

 COMPARATIVE PATHOLOGY: 

 REFERENCES:

  1. Abbondati E, Del-Pozo J, Hoather TM, Constantino-Casas F, Dobson JM. An immunohistochemical study of the expression of the hypoxia markers Glut-1 and Ca-IX in canine sarcomas.  Vet Pathol. 2013;50(6):1063-1069.
  2. Constantino-Casas F, Mayhew D, Hoather TM, Dobson JM. The clinical presentation and histopathologic-immunohistochemical classification of histiocytic sarcomas in the flat coated retriever.  Vet Pathol. 2011; 48(3):764-771.
  3. Ide T, Uchida K, Yumiko K, Kazuhiko S, Hiroyuki N. Pathological and immunohistochemical features of subdural histiocytic sarcomas in 15 dogs.  J Vet Diagn Invest. 2011; 23:127-132.
  4. Hao X, et al. The histopathologic and molecular basis for the diagnosis of histiocytic sarcoma and histiocyte-associated lymphoma of mice. Vet Pathol. 2010; 47: 434-445.
  5. Helie P, et al. Congenital cutaneous histiocytosis in a piglet. Vet Pathol. 2013; 51:812-815.
  6. Hirako A, Sugiyama A, Sakurai M, Ozaki K, Sakai H, Takeuchi T, Morita T, Moore PF. Cutaneous histiocytic sarcoma with E-cadherin expression in a Pembroke Welsh Corgi dog. J Vet Diagn Invest. 2015; 27(5):589-595.
  7. Kato Y, et al. The Class A macrophage scavenger receptor CD204 is a useful immunohistochemical marker of canine histiocytic sarcoma.  J Comp Path.  2013; 148:188-196.
  8. Maudlin EA, Peters-Kennedy J. Integumentary system. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 1. 6th Philadelphia, PA: Elsevier Saunders; 2016: 725-730.
  9. Moore PF. A review of histiocytic diseases of dogs and cats. Vet Pathol. 2014; 51(1):167-184.
  10. Ogihara K, Itoh T, Mizuno Y, Tamukai K, Madarame H. Disseminated histiocytic sarcoma in an African hedgehog (Atelerix albiventris). J Comp Pathol. 2016; [Epub ahead of print].
  11. Ong CB, Langohr IM. Pathology in practice. Histiocytic sarcoma. J Am Vet Med Assoc. 2015; 247(2):163-165.
  12. Pierezan F, Mansell J, Ambrus A, Rodrigues Hoffmann A. Immunohistochemical expression of ionized calcium binding adapter molecule 1 in cutaneous histiocytic proliferative, neoplastic and inflammatory disorders of dogs and cats. J Comp Pathol. 2014;151(4):347-351.
  13. Ramos-Vara JA, Miller MA. Immunohistochemical expression of E-cadherin does not distinguish canine cutaneous histiocytoma from other canine round cell tumors. Vet Pathol. 2011; 48(3):758-763.
  14. Soare T, Noble PJ, Hetzel U, Fonfara S, Kipar A. Paraneoplastic syndrome in haemophagocytic histiocytic sarcoma in a dog.  J Comp Path. 2012; 146:168-174.
  15. Thongtharb A, Uchida K, Chambers JK, Miwa Y, Murata Y, Nakayama H. Histological, and immunohistochemical features of histiocytic sarcoma in four domestic ferrets (Mustela putorius furo). J Vet Diagn Invest. 2016; 28(2):165-170.
  16. Valli VEO, Kiupel M, Bienzle D, Wood RD. Hematopoietic system. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 3. 6th Philadelphia, PA: Elsevier Saunders; 2016: 250, 253.
  17. van Kuijk L, van Ginkel K, deVos JP, et al. Peri-articular histiocytic sarcoma and previous joint disease in Bernese mountain dogs. J Vet Intern Med. 2013; 27:293-299.
  18. VanderHart DJ, Reese DJ, Greenberg SM. What is your diagnosis? Histiocytic sarcoma. J Am Vet Med Assoc. 2014; 244(8):899-901.
  19. Wong VM, Snyman HN, Ackerley C, Bienzle D. Primary nasal histiocytic sarcoma of macrophage-myeloid cell type in a cat.  J Comp Path. 2012; 147: 209-213.


Click the slide to view.



Click on image for diagnostic series.



Back | Home | Contact Us | Links | Help |