JPC SYSTEMIC PATHOLOGY
URINARY SYSTEM
January 2018
U-T04 (NP)

Signalment (JPC #1713077): Multiple male mice

HISTORY: An accident in the hallway of a laboratory animal facility resulted in the leakage of several bottles of chloroform. The following day many male mice housed in the facility were found dead.

HISTOPATHOLOGIC DESCRIPTION: Kidney: Multifocally proximal tubular epithelial cells are swollen with pale granular cytoplasm (degeneration) or cells are hypereosinophilic with retention of cytoplasmic detail and multifocal nuclear pyknosis or karyolysis (necrosis). The tubule epithelial cells in these areas are either attenuated or swollen with numerous intracytoplasmic, variably sized (up to 15um diameter) eosinophilic hyaline globules. Multifocally within the cortex there are moderate numbers of mildly dilated renal tubules that contain bright eosinophilic homogenous fluid (proteinosis) or granular eosinophilic cellular debris. Less affected cortical and medullary tubules are dilated and contain proteinaceous fluid. There is a focal aggregation of lymphocytes in the medulla.

MORPHOLOGIC DIAGNOSIS: Kidney, tubules: Degeneration and necrosis, acute, multifocal, moderate, with tubular proteinosis, mouse, rodent.

ETIOLOGIC DIAGNOSIS: Chloroform nephrosis

CAUSE: Chloroform (CHCI3)

GENERAL DISCUSSION: 

PATHOGENESIS: 

TYPICAL CLINICAL FINDINGS: 

TYPICAL GROSS FINDINGS: 

TYPICAL LIGHT MICROSCOPIC FINDINGS: 

ULTRASTRUCTURAL FINDINGS: 

DIFFERENTIAL DIAGNOSIS:

COMPARATIVE PATHOLOGY: 

REFERENCES: 

  1. Gathumbi PK, Mwangi JW, Mugera GM, Njiro SM. Toxicity of chloroform extract of Prunus Africana stem bark in rats: gross and histologic lesions, Phytother Res. 2002 May; 16(3):244-7.
  2. Haschek WM, Rousseaux CG, Wallig MA. Kidney and lower urinary tract. Fundamentals of Toxicological Pathology. 2nd ed. San Diego, CA: Academic Press; 2010:287.
  3. Miller MA, Zachary JF. Mechanisms and morphology of cellular injury, adaptation, and death. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th ed. Saint Louis, MO: Elsevier; 2017:13.
  4. Percy DH, Barthhold SW. Pathology of Laboratory Rodents and Rabbits. 4th ed. Ames, Iowa: Iowa State University Press; 2016:107-108.
  5. Sebastian MM, Baskin SI, Czerwinski SE. Renal toxicity. In: Gupta RC, ed. Veterinary Toxicology. 1st ed. San Diego, CA: Elsevier; 2007:162-163.
  6. Sehata S, et al. 26 week carcinogenicity study of chloroform in CB6F1 rasH2-transgenic mice. Toxicol Pathol. 2002 May-Jun;30(3):328-38.
  7. Schnellmann RG. Toxic responses of the kidney. In: Klaassen CD, ed. Casarett and Doull’s Toxicology, The Basic Science of Poisons. 6th ed. San Francisco, CA; McGraw-Hill; 2001:508.

 

 


Click the slide to view.



Click on image for diagnostic series.



Back | Home | Contact Us | Links | Help |