JPC SYSTEMIC PATHOLOGY
REPRODUCTIVE SYSTEM
January 2019
R-N02

Signalment (JPC #2329127): 12-year-old Belgian malinois military working dog

HISTORY:  None

HISTOPATHOLOGIC DESCRIPTION: Testicle and epididymis: Expanding and replacing normal testicular architecture and compressing surrounding seminiferous tubules is an unencapsulated, well demarcated, multilobulated densely cellular neoplasm composed of polygonal cells arranged in tubules, islands and cords which are separated and surrounded by dense bands of fibrous connective tissue.  Neoplastic cells are triangular to columnar, have indistinct cell borders, a moderate amount of eosinophilic granular cytoplasm with frequent 5 um clear vacuoles (lipid droplets), a round to oval nucleus with coarse chromatin and up to two distinct nucleoli.  Cells frequently palisade perpendicular to the basement membrane. Anisocytosis and anisokaryosis are mild. Mitoses average less than 1 per 40 x HPF.  Remaining seminiferous tubules are compressed at the periphery of the testicle and are diffusely atrophied, characterized by irregular undulant basement membranes and lack of developing sperm stages, with only sertoli cells remaining.  The epididymis exhibits a similar ductular atrophy with lack of mature sperm stages. 

MORPHOLOGIC DIAGNOSES: 

  1. Testicle: Sertoli cell tumor, Belgian malinois, canine.
  2. Testicle, seminiferous tubules; epididymis: Atrophy, diffuse, moderate, chronic with aspermatogenesis.

GENERAL DISCUSSION:

PATHOGENESIS:

TYPICAL CLINICAL FINDINGS:

TYPICAL GROSS FINDINGS: 

TYPICAL LIGHT MICROSCOPIC FINDINGS:

ULTRASTRUCTURE:

ADDITIONAL DIAGNOSTIC TESTS:

DIFFERENTIAL DIAGNOSIS:

Gross:  Tan to yellow-orange, discrete, and soft, with areas of hemorrhage

Microscopic:  Polygonal cells with abundant finely vacuolated eosinophilic cytoplasm and small round nuclei; prominent lipid accumulations; rare mitoses

Gross:  Pale to gray, soft; resemble malignant lymphoma cells; compresses adjacent parenchyma; bulging, lobulated and irregular

Microscopic:  Large, uniform, fairly discrete round cells, variable amounts of cytoplasm, large round nuclei, prominent nucleoli; arranged in a diffuse or intratubular pattern; multinucleate cells; lymphocytes; mitoses common; individual cell necrosis

Gross:  Gray, white or tan, firm, hemorrhage and necrosis in larger tumors

Microscopic:  Admixed neoplastic germ cells and Sertoli cells in tubular structures

Immunohistochemistry:  Sertoli cells are NSE and vimentin positive; germ cells are negative for NSE

COMPARATIVE PATHOLOGY:

REFERENCES:

  1. Ano H, Hidak Y, Katamoto H. Evaluation of anti-Müllerian hormone in a dog with a Sertoli cell Vet Derm. 2014;25:142-e41.
  2. Foster RA. Male reproductive system. In: McGavin MD, Zachary JF, eds. Pathologic Basis of Veterinary Disease. 6th ed. St. Louis, MO: Elsevier Inc; 2017:1024, 1211-1212.
  3. Foster RA. Male genital system. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 3. 6th ed.   Louis, MO: Elsevier Inc; 2016:494-495.
  4. Agnew DW, MacLachlan NJ. Tumors of the
  5. Patnaik AK, Mostofi FK. A clinicopathologic, histologic, and immunohistochemical study of mixed germ cell-stromal tumors of the testis in 16 dogs. Vet Pathol. 1993:(30)287-295
  6. Ramos-Vara JA, Miller MA. Immunohistochemical evaluation of GATA-4 in canine testicular tumors. Vet Pathol. 2009;46:893-896.


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