January 2018

Signalment (AFIP #1518769):  New Zealand Black (NZB) mouse


HISTOPATHOLOGIC DESCRIPTION:  M15a:  Kidney: Glomerular tufts diffusely and globally exhibit the following changes:  mild to moderate thickening of mesangial matrix and basement membranes by eosinophilic amorphous material (matrix), moderate hypercellularity, and hypertrophic visceral epithelial cells/podocytes.  Focally, glomeruli exhibit one or more of the following changes:  occasional karyorrhectic debris in place of mesangial and/or endothelial cells (lytic necrosis), multifocal adherence of tufts to Bowman’s capsule (synechia), mild thickening of Bowman’s capsule, hypertrophied parietal epithelial cells, and ectatic urinary spaces.  Multifocally, tubules are ectatic and occasionally contain proteinaceous eosinophilic material (proteinosis), sloughed epithelial cells (cellular casts) and/or mineral.  Tubular epithelial cells are often attenuated, and/or contain yellow-brown pigment, or many eosinophilic globules within the cytoplasm.  Plasma cells and lymphocytes are present multifocally in scattered small aggregates within the interstitium frequently around vessels.  The kidney surface is diffusely irregular and pitted. 

M15b:  Kidney (PAS):  The mesangium and basement membranes of glomerular capillaries, Bowman’s capsules, and occasional tubules are mildly thickened by PAS-positive material.

MORPHOLOGIC DIAGNOSIS:  Kidney:  Glomerulonephritis, membranoproliferative, chronic, diffuse, global, moderate, with glomerulosclerosis, and lymphoplasmacytic interstitial nephritis, NZB mouse, rodent.

Signalment (AFIP #1771042):  8-year-old mixed breed terrier

HISTORY:  Polyuria/polydipsia, lethargy and ascites.  Significant clinical laboratory data include: BUN-145; creatinine-4.5; total protein-3.6; albumin-1.1; calcium-7.2; urinary protein 3+; urine specific gravity-1.018; no significant sediment in urine.  No reference intervals are provided. 

HISTOPATHOLOGIC DESCRIPTION:  M15c:  Kidney:  Diffusely within the cortex, the glomeruli exhibit one or more of the following changes: diffusely and globally, the glomerular basement membrane (GBM) is thickened by a densely eosinophilic homogenous material (membranous change); glomerular tufts are occasionally enlarged with increased numbers of hypertrophic visceral epithelial cells; dilated uriniferous spaces; rare, small periglomerular interstitial aggregates of plasma cells and lymphocytes.  Renal tubular epithelium exhibit one or more of the following changes: mild tubular degeneration (swollen and vacuolated cytoplasm); necrosis (hypereosinophilic cytoplasm with nuclear pyknosis, karyorrhexis, or karyolysis); rare regeneration (basophilic cytoplasm with vesiculate nuclei, mitotic figures, piling up of epithelium);  intraluminal eosinophilic homogenous material (proteinaceous fluid) occasionally admixed with sloughed cellular and karyorrhectic debris (cellular casts); and tubular epithelial and luminal yellow-brown globular pigment (hemosiderin or lipofuscin)  

M15d:  Kidney (PAMS - silver):  Glomerular basement changes, including spikes and holes, associated with membranous glomerulonephropathy and best visualized with silver stain are subtle and inconsistent in this section 

M15e:  Kidney (Masson):  Diffusely there are regularly spaced red nodules along the abluminal surface of capillary walls (immune deposits)

MORPHOLOGIC DIAGNOSIS:  Kidney:  Glomerulonephritis, membranous, diffuse, global, moderate, with tubular degeneration and protein casts, terrier mix, canine.

ETIOLOGIC DIAGNOSIS: Immune-mediated glomerulonephritis (presumed, demonstration of immune complexes or complement required for definitive diagnosis)

CAUSE: Immune-mediated








contours of the GBM




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