JPC SYSTEMIC PATHOLOGY
REPRODUCTIVE SYSTEM
January 2019
R- F01

Signalment (JPC# 1421031):  Bovine fetus

HISTORY:  None

HISTOPATHOLOGIC DESCRIPTION (Slide a):  Chorioallantois:  Diffusely there is loss of chorionic villar epithelium of the cotyledon and replacement by abundant eosinophilic cellular and karyorrhectic debris (necrosis) admixed with few lightly basophilic, 2-4 um wide, infrequently septate hyphae that have parallel walls and dichotomous, right angle branching as well as fibrin, basophilic granular mineral and moderate numbers of lymphocytes, plasma cells, macrophages, and fewer neutrophils.  Multifocally within low to moderate numbers of sloughed trophoblasts and extracellularly in the necrotic debris there are colonies of 1-2um coccobacilli.  The chorioallantoic stroma is expanded by similar inflammatory cells with hemorrhage, fibrin, edema and scattered necrotic debris and mineral.  Multifocally within vessels there is disruption of the tunica intima and tunica media with replacement by cellular and karyorrhectic debris and rare fungal hyphae (necrotizing vasculitis).  Multifocally within the cellular debris on the allantoic membrane of the intercotyledonary chorioallantois, as well as enmeshed within fibrin and debris along the margin of the parietal yolk sac, there are many fungal hyphae.  The yolk sac stroma is infiltrated by neutrophils, lymphocytes and macrophages admixed with  small amounts of necrotic debris.

Haired skin:  There is multifocal mild epidermal hyperplasia, acanthosis, spongiosis and orthokeratotic hyperkeratosis admixed with few previously described fungal hyphae.  Diffusely, the superficial dermis is infiltrated by moderate numbers of lymphocytes, plasma cells and fewer macrophages, and there is separation of dermal collagen by increased clear space (edema).  There is a focal intra-corneal pustule composed of few degenerate neutrophils.  The follicle subjacent to this pustule is hyperkeratotic and contains a small amount of amorphous eosinophilic cellular debris and few degenerate neutrophils (folliculitis).

Slide b (Periodic Acid Schiff):  PAS positive fungal hyphae that are 2-4 um wide, infrequently septate,  parallel walled, and having dichotomous, right angle branching present on the allantoic membrane, parietal yolk sac within chorioallantoic stroma and vessels, and within the stratum corneum of the skin. 

MORPHOLOGIC DIAGNOSIS: 

  1. Placenta, chorioallantois: Placentitis, necrotizing, subacute, diffuse, moderate, with necrotizing vasculitis, coccobacilli and fungal hyphae, breed unspecified, bovine.
  2. Haired skin: Dermatitis, subacute, diffuse, moderate, with multifocal orthokeratotic hyperkeratosis and few superficial fungal hyphae. 

ETIOLOGIC DIAGNOSIS:  Mycotic placentitis and dermatitis

GENERAL DISCUSSION:

PATHOGENESIS:

TYPICAL GROSS FINDINGS:

TYPICAL LIGHT MICROSCOPIC FINDINGS:

ADDITIONAL DIAGNOSTIC TESTS:

DIFFERENTIAL DIAGNOSIS:

Both cotyledonary and intercotyledonary bovine placentitis:

Late term abortion in cattle:

Fetal dermatitis with multifocal dermal plaques:

Amniotic fluid changes: thick, white, yellow

COMPARATIVE PATHOLOGY:

REFERENCES:

  1. Allison N. Theriogenology question of the month. How would you confirm a diagnosis of mycotic infection in an aborted fetus? J Am Vet Med Assoc. 2004;225(6):849-851.
  2. Antoniassi NA, Juffo GD, Santos AS, Pescador CA, Ferreiro L, Driemeier D. Geotrichum candidum as a possible cause of bovine abortion. J Vet Diagn Invest. 2013 Nov;25(6):795-797.
  3. Foster RA. Male reproductive system. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th ed. St. Louis, MO: Elsevier; 2017:1181-1182.
  4. Hughes K, Mueller K. Pathologic lesions of mycotic pneumonia in an alpaca following third compartment ulceration. J Vet Diag Invest. 2008;20(5): 672-675.
  5. Johnson CT, Lupson GR, Lawrence KE. The bovine placentome in bacterial and mycotic abortions. Vet Rec. 1994;134(11):263-266.
  6. Knudtson WU, Kirkbride CA. Fungi associated with bovine abortion in the northern plains states (USA). J Vet Diag Invest. 1992;4(2): 181-185.
  7. Schlafer DH, Foster RA. Female genital system. In: Maxie MG, ed. Jubb, Kennedy, and Palmers Pathology of Domestic Animals. Vol 3. 6th ed. St. Louis, MO: Saunders Elsevier; 2016:418-419.


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