JPC SYSTEMIC PATHOLOGY

INTEGUMENTARY SYSTEM

November 2016

I-P15

 

Signalment (JPC # 2277382):  Two-year-old male boxer

HISTORY:  This dog was underweight and had hyperkeratosis of the planum nasale, ulceration of the oral mucocutaneous junction, and erythematous and edematous lesions on the feet.

HISTOPATHOLOGIC DESCRIPTION:  Slide A:  Diffusely infiltrating the dermis, extending to and elevating the mildly hyperkeratotic epidermis, surrounding and separating collagen bundles and adnexa, and multifocally extending deeply into the subcutis, are numerous protozoa-laden macrophages and plasma cells, fewer lymphocytes and eosinophils, and occasional multinucleated giant cells (Langhans type). Intrahistiocytic protozoal amastigotes are 2-3 um in diameter with clear cytoplasm and a single 1 um diameter basophilic nucleus. The superficial dermis is multifocally mildly expanded by edema and fibrin, and dermal collagen is multifocally smudged.  There is moderate fibrosis within the deep dermis and subcutis. There is multifocal minimal orthokeratotic hyperkeratosis.  Myocytes in the panniculus carnosus are often atrophied.

Slide B:  Haired skin and subcutis (Giemsa):  Within macrophages there are numerous 2-3 um diameter, deeply-purple amastigotes with clear cytoplasm and a 1 um diameter nucleus with a smaller adjacent, often perpendicular kinetoplast.

MORPHOLOGIC DIAGNOSIS:  Haired skin and subcutis, site not specified:  Dermatitis and panniculitis, granulomatous and plasmacytic, multifocal to coalescing, marked, with numerous intrahistiocytic amastigotes, boxer, canine.

ETIOLOGY:  Leishmania sp.

ETIOLOGIC DIAGNOSIS:  Cutaneous leishmaniasis

GENERAL: 

PATHOGENESIS AND LIFE CYCLE: 

TYPICAL CLINICAL FINDINGS: 

TYPICAL GROSS FINDINGS:

Cutaneous:

Visceral:

 TYPICAL MICROSCOPIC FINDINGS: 

Cutaneous:

Visceral:

ULTRASTRUCTURE: 

ADDITIONAL DIAGNOSTIC TESTS: 

 DIFFERENTIAL DIAGNOSIS: 

Gross:

Microscopic:

COMPARATIVE PATHOLOGY: 

REFERENCES: 

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  2. Baneth G, Solano-Gallego L. Leishmaniasis. In: Greene CE, ed. Infectious Diseases of the Dog and Cat, 4th ed. St. Louis, MO: Elsevier Saunders; 2012: 734-749.
  3. Cheville NF. Ultrastructural Pathology: An Introduction to Interpretation, 2nd ed. Ames, IA: Iowa State University Press; 2009: 535-536.
  4. Colombo S, Abramo F, Borio S, et al. Pustular dermatitis in dogs affected by leishmaniosis: 22 cases.  Vet Dermatol. 2016;27(1):9-e4.
  5. Costa FA, Prianti MG, Silva TC, et al. T cells, adhesion molecules and modulation of apoptosis in visceral leishmaniasis glomerulonephritis. BMC Infect Dis. 2010;10:112.
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  7. Ferro S, Palmieri C, Cavicchioli L, De Zan G, Aresu L, Benali SL. Leishmania amastigotes in neoplastic cells of 3 non-histiocytic canine tumors. Vet Pathol. 2013;50(5):749-752.
  8. Garcia-Martinez JD, Tvarijonaviciute A, Ceron JJ, Caldin M, Martinez-Subiela S. Urinary clusterin as a renal marker in dogs. J Vet Diagn Invest. 2012;24(2):301-306.
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  12. Koutinas AF, Koutinas CK. Pathologic mechanisms underlying the clinical findings in canine leishmaniasis due to Leishmania infantum/chagasiJ Vet Diagn Invest. 2014;26(2):286-90.
  13. Kumar V, Abbas AK, Aster JC, eds. Robbins and Cotran Pathologic Basis of Disease. 9th ed. Philadelphia, PA: Elsevier; 2015: 393-394.
  14. Márquez M, Pedregosa JR, López J, Marco-Salazar P, Fondevila D, Pumarola M. Leishmania amastigotes in the central nervous system of a naturally infected dog. J Vet Diagn Invest. 2013;25(1):142-146.
  15. Mauldin EA, Peters-Kennedy J. Integumentary system. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 1. 6th ed. Philidelphia, PA: Elsevier Saunders; 2015: 663-664.
  16. Miller WH, Griffin CE, Campbell KL. Viral, rickettsial, and protozoal skin diseases. In: Miller WH, Griffin CE, Campbell KL eds. Small Animal Dermatology. 7th ed. Philadelphia, PA: WB Saunders Company; 2013:354-357.
  17. Nascimento MS, Albuquerque TD, Nascimento AF, et al. Impairment of interleukin-17A expression in canine visceral Leishmaniosis is correlated with reduced interferon-y and inducible nitric oxide synthase expression. J Comp Pathol. 2015;153(4):197-205.
  18. Rosa FA, Leite HAC, Braga ET, et al. Cardiac lesions in 30 dogs naturally infected with Leishmania infantum chagasi Vet Pathol. 2014;51(3):603-6.
  19. Saridomichelakis MN, Koutinas AF. Cutaneous involvement in canine leishmaniosis due to Leishmania infantum (syn. chagasi). Vet Dermatol. 2015;26(1):69-70.
  20. Saridomichelakis M. Advances in the pathogenesis of canine leishmaniasis: epidemiologic and diagnostic implications. Vet Dermatol. 2009;20(5-6):471-489.
  21. Valli VEO, Kiupel M, Bienzle D. Hematopoietic system. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animal. Vol 3. 6th ed. Philidelphia, PA: Elsevier Saunders; 2015: 174-175.


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