November 2016



Signalment (JPC # 2277382):  Two-year-old male boxer

HISTORY:  This dog was underweight and had hyperkeratosis of the planum nasale, ulceration of the oral mucocutaneous junction, and erythematous and edematous lesions on the feet.

HISTOPATHOLOGIC DESCRIPTION:  Slide A:  Diffusely infiltrating the dermis, extending to and elevating the mildly hyperkeratotic epidermis, surrounding and separating collagen bundles and adnexa, and multifocally extending deeply into the subcutis, are numerous protozoa-laden macrophages and plasma cells, fewer lymphocytes and eosinophils, and occasional multinucleated giant cells (Langhans type). Intrahistiocytic protozoal amastigotes are 2-3 um in diameter with clear cytoplasm and a single 1 um diameter basophilic nucleus. The superficial dermis is multifocally mildly expanded by edema and fibrin, and dermal collagen is multifocally smudged.  There is moderate fibrosis within the deep dermis and subcutis. There is multifocal minimal orthokeratotic hyperkeratosis.  Myocytes in the panniculus carnosus are often atrophied.

Slide B:  Haired skin and subcutis (Giemsa):  Within macrophages there are numerous 2-3 um diameter, deeply-purple amastigotes with clear cytoplasm and a 1 um diameter nucleus with a smaller adjacent, often perpendicular kinetoplast.

MORPHOLOGIC DIAGNOSIS:  Haired skin and subcutis, site not specified:  Dermatitis and panniculitis, granulomatous and plasmacytic, multifocal to coalescing, marked, with numerous intrahistiocytic amastigotes, boxer, canine.

ETIOLOGY:  Leishmania sp.

ETIOLOGIC DIAGNOSIS:  Cutaneous leishmaniasis

















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