JPC SYSTEMIC PATHOLOGY
REPRODUCTIVE SYSTEM
January 2019
R-N05

Signalment (Slide A, JPC #2643312):  Dog

HISTORY:  None

HISTOPATHOLOGIC DESCRIPTION:  Ovary (per contributor):  Extending to submitted margins and diffusely effacing normal ovarian tissue is a multilobular, densely-cellular neoplasm composed of polygonal cells arranged in islands and cords which frequently palisade along the fibrovascular stroma forming rosette-like structures that surround a central eosinophilic secretory globule (Call-Exner bodies).  Neoplastic cells have distinct cell borders, a moderate amount of pale eosinophilic, granular to fibrillar cytoplasm, one to two round nuclei, finely-stippled chromatin and a distinct nucleolus.  The mitotic rate is 1 per 40x HPF.  Multifocally within the neoplasm there is fibrin, hemorrhage, edema and lakes of an eosinophilic acellular material (secretory product). 

MORPHOLOGIC DIAGNOSIS:  Ovary (per contributor):  Granulosa cell tumor, breed unspecified, canine.

Signalment (Slide B, JPC #1848611):  30-year-old female Bornean orangutan (Pongo pygmaeus)

HISTORY:  None

HISTOPATHOLOGIC DESCRIPTION:  Ovary:  Extending to submitted margins and diffusely effacing normal ovarian tissue is a multilobular, densely-cellular neoplasm composed of polygonal cells arranged in islands and cords and frequently palisade along the fibrovascular stroma forming rosette-like structures that surround a central eosinophilic secretory globule (Call-Exner bodies).  Neoplastic cells have indistinct cell borders, a scant amount of pale eosinophilic cytoplasm, one round to ovoid nucleus, finely-stippled chromatin and an indistinct nucleolus.  The mitotic rate is 1 per 40x HPF.  Multifocally, few lobules contain lakes of eosinophilic acellular material (secretory product).

MORPHOLOGIC DIANGOSIS:  Ovary:  Granulosa cell tumor, Bornean orangutan (Pongo pygmaeus), nonhuman primate.

Signalment (Slide C, JPC #4065007):  Horse

HISTORY:  None

HISTOPATHOLOGIC DESCRIPTION:  Ovary:  Diffusely expanding and effacing the ovarian tissue and extending to submitted margins is an unencapsulated, well demarcated, densely cellular neoplasm composed of neoplastic granulosa cells that line multiple variably sized tortuous cystic structures arranged in a macrofollicular pattern, or palisade along the margin of solidly cellular trabecular or tubule like structures on a moderate to dense, and often hemorrhagic, collagenous stroma.  Neoplastic cells have distinct cell borders, a moderate amount of pale eosinophilic to granular or vacuolated cytoplasm, a round to ovoid nucleus with finely-stippled chromatin and an indistinct nucleolus.  Anisocytosis and anisokarryosis are mild with 1 mitotic figure per 10 40X HPF.  Granulosa cells are surrounded by a layer of streaming spindle cells with indistinct cell borders, scant fibrillar eosinophilic cytoplasm, a round to oval nucleus with finely stippled chromatin and indistinct nucleoli (theca cells).  Admixed with theca cells and/or adjacent to the margins of follicular structures are aggregates of large polyhedral cells with variably distinct cell borders and abundant granular or vacuolated eosinophilic cytoplasm (resemble testosterone-producing Leydig cells).  Rarely, neoplastic granulosa cells surround pale eosinophilic secretory globules (Call-Exner bodies).  Cysts contain abundant hemorrhage or eosinophilic acellular material (secretory product).  Multifocally, the ovarian stroma contains hemosiderin laden macrophages and low numbers of lymphocytes, multifocal hemorrhage, fibrin and edema, and multiple ectatic lymphatic vessels.  Few macrophages contain phagocytized red blood cells.

MORPHOLOGIC DIAGNOSIS:  Ovary:  Granulosa cell tumor, breed unspecified, equine.

SYNONYMS: 

GENERAL DISCUSSION:

PATHOGENESIS:

TYPICAL CLINICAL FINDINGS:

TYPICAL GROSS FINDINGS:

TYPICAL LIGHT MICROSCOPIC FINDINGS:

ADDITIONAL DIAGNOSTIC TESTS:

DIFFERENTIAL DIAGNOSIS:

COMPARATIVE PATHOLOGY:

REFERENCES:

  1. Agnew DW, MacLachlan NJ. In: Tumors of the genital system. In: Meuten DJ, ed. Tumors in Domestic Animals. 5th ed. Ames, IA: John Wiley & Sons, Inc.; 2017:690-698.
  2. Banco B, Antuofermo E, Borzacchiello G, Cossu-Rocca P, Grieco V. Canine ovarian tumors: an immunohistochemical study with HBME-1 antibody. J Vet Diagn Invest. 2011;23(5):977-981.
  3. Barthold SW, Griffey SM, Percy DH. Pathology of Laboratory Rodents and Rabbits. 4th ed. Ames, IA: Blackwell Publishing; 2016:115, 210, 251,
  4. Buijtels JJ, de Gier J, Kooistra HS, Kroeze EJ, Okkens AC. Alterations of the pituitary-ovarian axis in dogs with a functional granulosa cell tumor. Theriogenology. 2010;73(1):11-19.
  5. Crabtree J. Review of seven cases of granulosa cell tumour of the equine ovary. Vet Rec. 2011;169(10):251.
  6. Durkes A, Garner M, Juan-Salles C, Ramos-Vara J. Immunohistochemical characterization of nonhuman primate ovarian sex cord-stromal tumors. Vet Pathol. 2012;49(5):834-838.
  7. Fernandes TR, Grandi F, Monteiro LN, Salgado BS, Rocha NS. What is your diagnosis? Unilateral ovarian mass in a mare. Vet Clin Pathol. 2011;40(3):399-400.
  8. Foster RA. Female reproductive system and mammae. In: McGavin MD, Zachary JF, eds. Pathologic Basis of Veterinary Disease. 6th ed. St. Louis, MO: Elsevier; 2017:1161-1162.
  9. Guzman-Silva MA, Costa-Neves M. Incipient spontaneous granulosa cell tumor in the gerbil, Meriones unguiculatus. Lab Anim. 2006;40(1):96-101.
  10. Jamieson S, Fuller PJ. Molecular pathogenesis of granulosa cell tumors of the ovary. Endocr Rev. 2012;33(1):109-144.
  11. Kennedy PC, Cullen JM, Edwards JF, et al. Histological Classification of Tumors of the Genital System of Domestic Animals. 2nd series. Volume IV. Washington, DC: Armed Forces Institute of Pathology; 1998.
  12. Masserdotti C, De Lorenzi D, Gasparotto L. Cytological detection of Call-Exner bodies in Sertoli cell tumors from 2 dogs. Vet Clin Pathol. 2008;37(1):112-114.
  13. McInnes ER, Ernst H, Germann PG. Spontaneous neoplastic lesions in control Syrian hamsters in 6-, 12-, and 24-month short-term and carcinogenicity studies. Toxicol Pathol. 2013;41(1):86-97.
  14. Schlafer DH, Foster RA. Female genital system. In: Maxie MG, ed. Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals. Vol 3. 6th ed. Louis: Elsevier; 2016:375-379.
  15. Smith LN, Rotstein DS, Ball RL, et al. Reproductive neoplasms in wild and long-term captive female Florida manatees (Trichechus manatus latirostris). J Zoo Wildl Med. 2015;46(4):895-903.
  16. Spoor MS, Flesner BK, Trzil JE, et al. What is your diagnosis? Intra-abdominal mass in a female spayed dog. Vet Clin Path. 2014;43(1):109-110.


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